Composite

Part:BBa_K2209002:Design

Designed by: Qinyu Ge   Group: iGEM17_Nanjing_NFLS   (2017-10-18)


Fused strain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 165
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1134
    Illegal BsaI.rc site found at 1720
    Illegal SapI site found at 4799


Design Notes

Expressed protein in E. coli. were obtained from the modified gene, while whether it could express well in P.putida and have function which degrade aniline and it analogue. It could be verified by the triphiles of bacteria.


Source

P.putida


References